Dược động học theo con đường tiêm màng bụng và con đường cho ăn cửa Catechin (Epigallocatechin gallate) ở cá Yellowtail (Seriola quinqueradiata)

The reactive oxygen species (ROS) have been generated by Chattonella spp., may be one of the  causative  factors  responsible  for  the  toxic  effects  to  living  organisms,  especially  to yellowtail (Seriola quinqueradiata). On the other hand, (-)-Epigallocatechin gallate (EGCG), known as one of the most powerful green tea polyphenol antioxidants in vitro and in vivo, was thought to be an effective solution for protection of the damage by Chattonella spp. in fish. Therefore, the present study aimed to obtain pharmacokinetics of EGCG by oral gavage and intra-peritoneal (IP) in yellowtail. Blood and tissue samples were collected and analyzed by HPLC assay. Pharmacokinetic parameters of EGCG in fish plasma were obtained by fitting 

with 1-compartment IP administration model. The result showed that EGCG was present in all plasma samples collected at 2-h post-dose (2.22 ± 1.46 μg/mL), then decreased quickly in groups  of  4-h  and  8-h  administration  (4.37  ±  3.98  μg/mL  and  0.99  ±  0.66  μg/mL, respectively)  and  not detected  in  late-collected  groups  in  IP  administration;  whereas,  there were only one plasma specimen detected EGCG (0.623 μg/mL, at 2-h post-treatment) in oral gavage  and  no  detection  of  EGCG  in  all  muscle  and  liver  samples.  In  pharmacokinetic parameters, there were a high elimination rate constant (0.24) and a short half-life (2.89 h). It suggested  that  EGCG  was  poorly  absorbed  and/or  quickly  eliminated  in  yellowtail.  Our results provide a basis for understanding about pharmacokinetics of EGCG in fish.