So sánh các phương pháp phá huỷ thành tế bào vi khuẩn Streptococcus iniae sử dụng trong quá trình phân tách Protein bằng SDS-PAGE

Streptococcus  iniae  is  a  recognised  pathogen  of  hemorrhage  impacting  aquaculture production  of  Asean  seabass  Lates  calcarifer  (Bloch,  1790).  However,  it  is  difficult  when analyses of bacterial antigens and the immune response after vaccination because S. iniae is a Gram-positive  bacteria  and  the  cell  wall  had  to  be  degraded  before  processing  for  SDS-PAGE. Different methods for degradation the cell wall of S. iniae isolates to perform protein profiles  by  using  SDS-PAGE  were  evaluated.  Characterize  isolates  of  S.  iniae  from  Lates calcarifer  perform  the  degradation  processes  included  treatment  with  acetone,  lysine, mutanolysine as well as sonication. Each of the degradation process was performed separately or in combinations of sonication. Among the methods had been tested, degradation process by acetone,  lysine,  sonication  or  combination  showed  unclearly  band  or  no  band  on  the electrophoresis gel. Meanwhile, using mutanolysine performed best result with fully detected protein profile as clearly bands between 10 to 100 kDa.