This current research compares the biochemical composition of the black seahorse (Hippocampus kuda) processed by three different methods: freeze-dried, oven-dried at 40oC and soaked in 45º rice whisky. Also, it evaluates the effects of using seahorse powder or seahorse wine on both quality and quantity of sperm of the Swiss mouse (Mus musculusdomesticus). Results showed that the total amount of amino acids was four folds higher (P<0.05) in freeze-dried seahorse compared with those dried at 40oC or soaked in 45º rice whisky for two days. Significant changes of some amino acids and fatty acids were recorded in the seahorses soaked in 45º rice whisky. Feeding male mouse daily with 400 mg of seahorse powder or 0.05 ml of seahorse wine for 10 continuous days significantly increased both sperm density and number of active sperms (P<0.05). Seahorse wine was found much more effective than seahorse powder regarding stimulating sperm production. It is recommended that more works should be done on changes of the biochemical composition of seahorses soaked in rice whisky, which may shed the light on pharmaceutical properties of the black seahorse.