Nghiên cứu đặc tính lý hóa học của tinh trùng cá chẽm mõm nhọn Psammoperca waigiensis

The  objective  of  the  present  study  was  to  determine  the  physic-biochemical  propreties  of sperm of Waigeu seaperch Psammoperca waigiensis. The biochemical properties of seminal fluid  and  the  physical  properties  of  sperm  were  analyzed.  The  seminal  plasma  contained 154.45±2.53  mM  sodium  (Na),  16.89±0.98  mM potassium  (K),  113.58±1.19  mM  chlorine (Cl),  12.75±1.19  mM  calcium  (Ca)  and  6.58±0.70  mM  magnesium  (Mg).  The  following organic component was found: total protein 1.15±0.25 (g.dl-1). The mean sperm density was estimated to be 31.35±2.12 (x10 9 spermatozoa.l-), spermatocrit (%) ranged from 84 to 92 in sperm samples and the mean was 87.7±2.54 (%). The mean of milt volume was 1.28±0.15 ml.fish-1.  Beside,  sperm  movement  was  also  analyzed  included:  motility  of  sperm  (%), velocity (µm.s-1), duration (s). The result was reported to be 95.80±1.23% for sperm motility, 50±3.03 µm.s-1 for the velocity and 18.58±15.66 s for duration of sperm. 

 

Highly significant relationships were found between sperm density and spermatocrit (P<0.01) indicating that optical density can be used as a quick and accurate method of estimating sperm density.  Beside,  the  number of  sperm  was  significantly  relationship with  volume of  semen (P<0.01).  A  significant  correlation  was  also  observed  between  Ca  of  seminal  plasma  and motility sperm and duration (P<0.05), between Na of seminal plasma and velocity (P<0.05). These  parameters  should  be  considered  when  developing  procedures  for  either  artificial fertilization or for cryopreservation of sperm.