The artificial sex change in humphead wrasse (Cheilinus undulatus) was examined. Nine of individuals of mean body weight (BW) 3.2 ± 0.2 kg were treated with 17α – Methyltestosterone (MT). MT was administered intramuscularly three times at the dose of 0 mg.kg-1 (Treatment 1 – T1), 5 mg.kg-1 (Treatment 2 – T2), and 10 mg.kg-1 (Treatment 3 – T3) every 30 days. After 60 and 120 days of injections, the gonad sections of all fish sampled from T2 and T3 were observed. The result showed that spermatocytes were mostly detectable in the gonads of two-thirds of experimented fish of T2 and T3 after 60 days of injection. 100% of fish of these two treatments were shown to have gonads with spermatid after 120-day injection. In T2 (5 mg.kg-1) there were two fish with sperm in the gonads, higher than T3 (10 mg.kg-1), with only one fish. In control treatment (T1), however, only oocytes of phases II, III were found in the gonads. The result also indicated that induction of female-to-male sex inversion in juvenile Cheilinus undulatus by MT (5 and 10 mg.kg-1) within 180 days of injection was completely effective with white milt.